Posted on Mar.29, 2012, under Veterinary Equipment
The Flu Detect™ Test Strip is an in-vitro, rapid immunochromatographic assay designed to aid in the qualitative detection of Influenza type A virus in tracheal and cloacal specimen from symptomatic birds or flocks. This assay detects all 15 subtypes of Influenza type A virus.
Influenza type A viruses can infect avian, porcine, equine and other species including humans. Infected birds can shed influenza virus in their saliva, nasal secretions, and feces. Domesticated birds may become infected with avian influenza virus through direct contact with infected waterfowl or other infected poultry, or through contact with surfaces or materials that have been contaminated with the virus.
Disease signs in poultry range from only a slight decrease in egg production to a highly fatal fulminating infection. Signs of infection may include respiratory problems, edema of the head and face or diarrhea. The clinical signs are very variable and are influenced by factors such as the virulence of the infecting virus, species affected, age, sex, concurrent diseases and environment.
In broilers, the signs of disease are frequently less obvious with severe depression, lack of appetite, a marked increase in mortality, Edema of the face and neck and neurological signs such as torticollis and ataxia. Avian influenza is characterised histologically by vascular disturbances leading to edema, haemorrhages and perivascular cuffing, especially in the myocardium, spleen, lungs, brain and wattles. Birds that die of peracute disease may show minimal gross lesions, consisting of dehydration and congestion of viscera and muscles.
This product is developed based on Rapid Immuno-Migration (RIM) technology. The test strip uses two antibodies that are specific to Influenza type A virus. Tracheal or oropharyngeal samples should be taken from behind the tongue and into the trachea or oropharyngeal area. Cloacal samples should be taken from within the cloacal area avoiding excess solid fecal material or visible blood. If samples will not be tested immediately they should be stored at 4°C for up to 24 hours. For prolonged storage, samples should be kept frozen (-20°C). Flu Detect™ test kits should be stored between 2 and 30°C (35-86°F).
After 15 minutes, observe the presence or absence of pink/purple bands in the center of the test strip between the two absorption pads. The control band appears in the upper end of the test strip (closest to the handle), while the sample test results are read in the lower part of the test strip. Positive results should be submitted to a reference lab for confirmation and subtype determination. Negative results indicate that no detectable Influenza type A virus is present. Contact the manufacturer